CRISPR Technologies A Tool For Engineering Microbes

CRISPR-CAS9 TECHNOLOGY OVERVIEW CRISPR-Cas9 biotechnology is now extensively applied across multiple disciplines, including basic sciences, food/crop development, fuel generation, drug development, and human genome engineering (22). Naturally occurring crRNA-tracrRNA complexes of type II CRISPR systems may be synthetically engineered into chimeric single-guide RNAs (sgRNAs), which are programable fusion molecules (23). The development of guide RNAs…...